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Item #:
79847519
Media - Trypsin EDTA 1x 0.05% Trypsin0.53 mM EDTA in HBSS wOut Calcium and Magnesium- 6 x 100 mL, CS
Item #:
79847519
BZD 508
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What Stands Out
Ready-to-Use Solution
Conveniently pre-prepared for immediate use, this media minimizes preparation time, ensuring efficient cell dissociation and subculturing without the hassle of mixing components.
Calcium and Magnesium-Free
Free from calcium and magnesium, this formulation prevents unwanted cell aggregation, enhancing cell yield and viability during your experiments, making it ideal for sensitive cell types.
Optimal Cell Handling
Containing 0.05% trypsin and 0.53 mM EDTA, it efficiently detaches cells while maintaining high viability, providing researchers with reliable results and predictable cell culture outcomes.
Product Details
| Item Weight | 1.5 lbs (680 grams) |
Who Should Buy?
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Cell Culture
Essential for enzymatic cell detachment and passaging in various cell culture applications in laboratory settings.
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Research Labs
Ideal for researchers working with adherent cell lines who require reliable trypsinization for experiments.
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Biotechnology
Beneficial for bioprocessing and biomanufacturing, where efficient cell detachment is crucial for downstream applications.
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Non-Adherent Cells
Not suitable for cell types that do not adhere to surfaces, as it may not provide effective detachment.
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Sensitive Cell Lines
Not recommended for sensitive or fragile cell lines that may be damaged by trypsinization processes.
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Calcium/Magnesium Dependent
Unsuitable for experiments that require calcium and magnesium, as these essential ions are absent in the formulation.
Product Description
Media - Trypsin EDTA 1x 0.05% Trypsin0.53 mM EDTA in HBSS wOut Calcium and Magnesium- 6 x 100 mL, CS
About This Item
Introducing Media - Trypsin EDTA 1x 0.05% Trypsin 0.53 mM EDTA in HBSS wOut Calcium and Magnesium - 6 x 100 mL Are you looking for a reliable and efficient solution for cell detachment and dissociation? Look no further than Media - Trypsin EDTA. This high-quality trypsin EDTA media offers a convenient and effective way to detach and harvest cells for your cell culture needs. Manufactured to meet the highest standards of quality and purity, Media - Trypsin EDTA provides optimal conditions for cell detachment, ensuring a seamless cell passaging and subculture process. With its 1x concentration of 0.05% trypsin and 0.53 mM EDTA, this media offers a gentle yet effective enzymatic activity, ensuring minimal damage to your cells during the dissociation process. Whether you're working in a research laboratory or a biotechnology facility, Media - Trypsin EDTA is designed to meet your specific needs.
Its working concentration has been carefully formulated to ensure maximum efficiency and viability of your cells. Additionally, it is supplied in a convenient 6 x 100 mL package, providing ample supply for your cell culture requirements. What sets Media - Trypsin EDTA apart is its comprehensive functionality and versatility. Its specialized formulation makes it suitable for a wide range of applications, including tissue culture, cell counting, cell morphology analysis, and more.
You can trust this trusted brand to deliver consistent and reliable results every time. When it comes to cell culture, precision and accuracy are key. That's why you can rely on Media - Trypsin EDTA to provide you with the highest quality products. Don't settle for anything less than the best when it comes to your research or laboratory work. Choose Media - Trypsin EDTA for all your cell detachment and dissociation needs.
Experience the difference that a premium product can make in your research outcomes. Order yours today and take your cell culture experiments to the next level.
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Customer Questions & Answers
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Question:
What is Trypsin EDTA and its primary use?
Answer: Trypsin EDTA is an enzyme solution that aids in cell detachment during cell culture processes. It primarily provides a safe environment for cells by reducing cellular adhesion, allowing for efficient passaging of adherent cells in a laboratory setting. It is commonly used in molecular biology, tissue culture, and biotechnological applications where maintaining viable cell populations is critical. -
Question:
What is the significance of EDTA in Trypsin EDTA solution?
Answer: EDTA (Ethylenediaminetetraacetic acid) is included in Trypsin EDTA solutions to chelate calcium and magnesium ions, which are critical for cell adhesion. By binding these ions, EDTA enhances the efficacy of trypsin in dissociating cells from the culture surface, thus facilitating easier and gentler cell harvesting for subculturing or experimentation. -
Question:
How should I store Trypsin EDTA solution?
Answer: Trypsin EDTA solutions should be stored at -20°C for long-term preservation and should be kept away from light. It's important to avoid repeated freeze-thaw cycles, as this can affect the enzyme's activity. For immediate use, you may store it in the refrigerator for a short period. Proper storage ensures that the solution remains effective for optimal cell culture results. -
Question:
What cell types can benefit from Trypsin EDTA?
Answer: Trypsin EDTA is suitable for a wide range of adherent cell types, including but not limited to fibroblasts, epithelial cells, and some types of stem cells. It allows researchers to detach and process these cells effectively, ensuring they remain viable during passaging or experimentation. The choice of cell type should be validated, as variations exist in adhesion properties and sensitivity. -
Question:
Is Trypsin EDTA suitable for sensitive cell lines?
Answer: Yes, Trypsin EDTA can be used for sensitive cell lines, but caution is advised. The concentration of trypsin and duration of activity should be optimized to minimize cellular stress. Using lower concentrations of trypsin or reducing exposure time can help protect sensitive cell types while effectively dissociating them from the culture surface. -
Question:
Can I use Trypsin EDTA for primary cell cultures?
Answer: Trypsin EDTA can be employed for primary cell cultures, but the protocol should be tailored for the specific cell type. Primary cells often require gentler handling, so adjustments in trypsin concentration or incubation time can be advantageous. It is essential to monitor the cells during the detachment process to avoid damaging them, ensuring successful downstream applications. -
Question:
What are the safety considerations when using Trypsin EDTA?
Answer: When using Trypsin EDTA, it is essential to handle the solution in a biological safety cabinet and use appropriate personal protective equipment (PPE) such as gloves and goggles. Trypsin is a proteolytic enzyme that can cause skin and eye irritation. Therefore, proper lab safety protocols should always be followed to minimize any risk to laboratory personnel. -
Question:
How can I optimize Trypsin EDTA use in my experiments?
Answer: To optimize Trypsin EDTA usage, consider conducting a time-course experiment to assess the optimal detachment time for your specific cell type. Adjusting both trypsin concentration and incubation time can help determine the best conditions for maintaining cell viability. Also, a quick rinse of the culture with PBS prior to applying Trypsin EDTA can enhance results by removing excess media. -
Question:
How do I dilute Trypsin EDTA for use?
Answer: To dilute Trypsin EDTA, it is crucial to follow a protocol that complements your cell type's needs. Generally, a 1x solution is prepared by diluting a concentrated stock solution with sterile phosphate-buffered saline (PBS) or cell culture media, ensuring proper mixing. The dilution should be performed in a sterile environment to maintain sterility and avoid contamination. -
Question:
Where can I buy Media - Trypsin EDTA 1x 0.05% Trypsin0.53 mM EDTA in HBSS wOut Calcium and Magnesium- 6 x 100 mL, CS in Belize?
Answer: You can purchase Media - Trypsin EDTA 1x 0.05% Trypsin0.53 mM EDTA in HBSS without Calcium and Magnesium from Ubuy, a reliable online platform known for offering a range of lab products. On Ubuy, customers can easily find this product with additional details and options tailored for laboratory needs in Belize.
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Features & Benefits
- Size: 6 x 100 mL
- 0.05% Trypsin/0.53 mM EDTA in HBSS without sodium bicarbonate, calcium, and magnesium
- Used to release adherent cells from tissue culture plates
- Enhances trypsin efficacy by sequestering divalent cations
- Complete Certificate of Analysis available for each production lot
- Full formulation information included
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